Propofol Inhibits Lipopolysaccharide-Induced Tumor Necrosis Factor-Alpha Expression and Myocardial Depression through Decreasing the Generation of Superoxide Anion in Cardiomyocytes
Figure 2
The inhibitory effect of propofol on gp91phox protein and mRNA expression in LPS-stimulated cardiomyocytes. Cardiomyocytes were pretreated with 10% intralipid or propofol (50 μM) followed by LPS (4 μg/mL) for 2 or 4 hours. (a) gp91phox protein was determined by western blot analysis at 4 hours after LPS treatment. (b) Gray intensity analysis of the western blot results of four groups. (c) Real time RT-PCR amplification for gp91phox mRNA (each bar represents the mean ± S.D, , compared with control group; , compared with LPS group; ).
