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Oxidative Medicine and Cellular Longevity
Volume 2014 (2014), Article ID 161942, 10 pages
http://dx.doi.org/10.1155/2014/161942
Research Article

Sildenafil Attenuates Hepatocellular Injury after Liver Ischemia Reperfusion in Rats: A Preliminary Study

1Experimental Surgical Unit, Aretaieion University Hospital, 11528 Athens, Greece
2Second Department of Surgery, Aretaieion University Hospital, 11528 Athens, Greece
3Pathology Department, Sismanoglio General Hospital, 15126 Athens, Greece
4Department of Anesthesiology, Penteli Pediatric Hospital, 15236 Athens, Greece
5Fourth Department of Surgery, Attikon University Hospital, 12462 Athens, Greece
6Department of Anesthesiology, Aretaieion University Hospital, 11528 Athens, Greece

Received 28 March 2014; Revised 16 May 2014; Accepted 19 May 2014; Published 4 June 2014

Academic Editor: Dimitrios Tsikas

Copyright © 2014 Spyridon Savvanis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We evaluated the role of sildenafil in a rat liver ischemia-reperfusion model. Forty male rats were randomly allocated in four groups. The sham group underwent midline laparotomy only. In the sildenafil group, sildenafil was administered intraperitoneally 60 minutes before sham laparotomy. In the ischemia-reperfusion (I/R) group, rats were subjected to 45 minutes of hepatic ischemia followed by 120 minutes of reperfusion, while in the sild+I/R group rats were subjected to a similar pattern of I/R after the administration of sildenafil, 60 minutes before ischemia. Two hours after reperfusion, serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured and histopathological examination of the lobes subjected to ischemia as well as TUNEL staining for apoptotic bodies was performed. Additionally, myeloperoxidase (MPO) activity and the expression of intercellular adhesion molecule-1 (ICAM-1) were analyzed. Serum markers of hepatocellular injury were significantly lower in the sild+I/R group, which also exhibited lower severity of histopathological lesions and fewer apoptotic bodies, as compared to the I/R group. The I/R group showed significantly higher MPO activity and higher expression of ICAM-1, as compared to the sild+I/R group. Use of sildenafil as a preconditioning agent in a rat model of liver I/R exerted a protective effect.