Oxidative Medicine and Cellular Longevity / 2014 / Article / Fig 2

Research Article

Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells

Figure 2

Apoptosis induction by ATRA and/or DAC + SAHA treatment: HL-60 cells were treated for 48 h by 1 μM ATRA and/or DAC + SAHA combination. (a) Cell viability assessed by MTT test. Error bars represent ±SEM of at least 5 measurements. Statistical significance degree of difference between treated samples and the corresponding control: (*), (**), (***). (b) Fraction of cells in subG1 phase (dotted columns); PARP fragmentation: f89/f116 ratio (checked columns); Annexin V+/PI-fraction of Annexin V-FITC/PI stained cells (hatched columns). Error bars represent ±SEM of at least 3 measurements. (c) Immunoblots showing PARP fragmentation and caspase-3 activation after 48 h ATRA and/or DAC + SAHA treatment in HL-60 cells and β-actin expression as a loading control. Images are representatives of at least three experiments.