Oxidative Medicine and Cellular Longevity / 2014 / Article / Fig 5

Research Article

Decitabine and SAHA-Induced Apoptosis Is Accompanied by Survivin Downregulation and Potentiated by ATRA in p53-Deficient Cells

Figure 5

(a) Fraction of non-subG1 cells in G2/M cell cycle phase monitored by PI-staining of ethanol-fixed cells treated by 1 μM ATRA, DAC + SAHA, or DAC + SAHA + ATRA combination. Data are averaged from at least three experiments. Statistical significance degree between corresponding samples was analyzed and value evaluated as (*), (**), (***). (b) Analysis of survivin (AlexaFluor488, green) and (c) BAX (AlexaFluor647, red) expression and localization in DAC + SAHA and/or ATRA-treated HL-60 cell line by confocal microscopy and (d) flow cytometry. In (c), mitochondria are visualised by COX IV (AlexaFluor488, green) and nuclear DNA stained by Hoechst 33342 (blue). Scale bar in microscopy images represents 5 μm.