Leptin Level and Oxidative Stress Contribute to Obesity-Induced Low Testosterone in Murine Testicular Tissue
Figure 4
Electron microscopy of mice testes following the diet-induced fat for 8 weeks and 19 weeks shows a large number of lipid droplets, irregular karyotype, and heterochromatin side set (arrow) of Leydig cells ((e), (f), (h), and (i)) compared to control ((d) and (g)). (a) Normal sperm formation in the control group, (b) normal morphology of basement membrane, spermatogonia, and primary spermatocytes in the DIO group, (c) normal morphology of spermatids, (d) normal stromal cells and blood vessels, (e) in Leydig cells: a large number of lipid droplets, irregular karyotype, and heterochromatin side set found in DIO group at 8 weeks, (f) in Leydig cells: a large number of lipid droplets, irregular karyotype, and heterochromatin side set found in DIO-R group at 8 weeks, (g) normal Leydig cells, a small amount of fat vacuolar found in control group at 19 weeks, (h) in Leydig cells: a large number of lipid droplets, irregular karyotype, and heterochromatin side set found in DIO group at 19 weeks, and (i) in Leydig cells: irregular karyotype and heterochromatin side set found in DIO-R group at 19 weeks.
