Effects of Paracetamol on NOS, COX, and CYP Activity and on Oxidative Stress in Healthy Male Subjects, Rat Hepatocytes, and Recombinant NOS
Figure 7
Effect of paracetamol (APAP) at 100 μM (15 mg/L) on the peak area ratio of m/z 47 for [15N]nitrite to m/z 46 for [14N]nitrite (a) and on the peak area ratio of m/z 63 for [15N]nitrate to m/z 62 for [14N]nitrate (b) upon incubation of adult rat hepatocytes with L-[guanidine-15N2]-arginine (5 mM) in the absence and in the presence of LiCl (1 mM) for the indicated times at 37°C as described elsewhere [8]. Reactions were terminated by addition of 400 μL aliquots of ice cold acetone and samples were further processed for GC-MS analysis. Data are shown as mean ± SD from three independent experiments; no statistical analysis was performed.
