Resveratrol Counteracts Inflammation in Human M1 and M2 Macrophages upon Challenge with 7-Oxo-Cholesterol: Potential Therapeutic Implications in Atherosclerosis
Figure 1
Flow cytometric analysis of differentiation and activation surface markers on M1 and M2 macrophage subsets. Resveratrol prevented 7-oxo-cholesterol (7oxo-C) induced CD16 and CD14 changes in M1 (a) and M2 (b) macrophage subsets. Polarized M1 and M2 macrophages pretreated or not with resveratrol for 1 hour were stimulated with 7oxo-C (15 μM) for 20 hours and then analyzed for surface molecule expression by flow cytometry. Macrophages stimulated with LPS (100 ng/mL) were used as positive control. The results of one representative experiment of three are shown. The number in the histograms shows the mean fluorescence intensity.
