Resveratrol Counteracts Inflammation in Human M1 and M2 Macrophages upon Challenge with 7-Oxo-Cholesterol: Potential Therapeutic Implications in Atherosclerosis

Figure 3

Gel zymography for MMP-2 and MMP-9 detection. Pretreatment of cells with resveratrol prevented upregulation of MMP-2 (a) in M1 and M2 subsets and of MMP-9 (b) in M2 macrophages in response to 7-oxo-cholesterol (7oxo-C). Culture supernatants of polarized M1 (□) and M2 (■) macrophages treated or not with resveratrol (Resv; 30 μM) for 1 hour and then stimulated with 15 μM 7oxo-C for 20 hours or left unstimulated were subjected to acrylamide gel electrophoresis and the gelatinolytic activity was determined by classical zymography as described in Section 2. Results are expressed as means ± SD of four independent experiments (^∗𝑃<0.001; ^†𝑃<0.05). Representative Western blotting and zymograms are reported on the top of the bar plot.

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