Research Article

Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation

Figure 6

(a): Western blot results of Nrf2 expression in both the cytoplasm and nucleus from all three groups. Vitamin A significantly increased the level of Nrf2 in nucleus and decreased the level of Nrf2 in cytoplasm. PCNA and β-actin were used as protein loading control. (b): Gel shift assay showing Nrf2-ARE-binding activity was higher after BDL and much higher after treatment with vitamin A. SHAM group ( ), BDL group ( ), BDL + vitA group ( ). Data are presented as mean ± SD. versus SHAM group; versus BDL group.