Table 1: (a) PCR primer sequences. (b) Information regarding antibodies employed in Western analysis.
(a)

Gene nameSenseAntisenseSize (bp)

BMP-2 5′-TATATGCTCGACCTGTACCG-3′5′-CTTCCTGCATTTGTTCCCGA-3′247
Myosin Va 5′-CACCTACGGAACCCTGACAT-3′5′-TGCAAAGATGTGAGGGTCCA-3′243
Myosin Vb 5′-GGCTGAACTAACCAAGGACT-3′5′-GGCAACAAGCACAATTCCAC-3′256
Myosin Vc 5′-GAAGATGGAAGGAAAGCAGG-3′5′-TCCTCAAGATCCCTGTTTTC-3′255
SPP1 5′-TGGCTTACGGACTGAGGTCA-3′5′-GACCTCAGAAGATGAACTC-3′486
MGP 5′-ATCCTGGCTGCGCTGGCCGTG-3′5′-GAAGTAGCGGTTGTAGGCGGC-3′264
Sma22 5′-AAGCCAGTGAAGGTGCCTGAG-3′5′-TTGAAGGCCAATCACGTGCTT-3′311
Fstl1 5′-GTGGCAGTAATGGCAAGAC-3′5′-GTACTTGTCTAGGATCTCAC-3′260
GAPDH 5′-AAGAAGGTGGTGAAGCAGG-3′5′-ACCCTGTTGCTGTAGCCATA-3′197

(b)

NameVenderCat. no.SpeciesDilution

BMP-2BioworldBS3473Rabbit monoclonal IgG1 : 500
MYO5aSigmaSab2501441Goat polyclonal IgG1 : 1000
Smad1Cell Signaling9516Rabbit monoclonal IgG1 : 1000
Sm22EpitomicsS2112Rabbit polyclonal IgG1 : 1000
OPNBioworldBS1264Rabbit monoclonal IgG1 : 1000
MGPAbgentAp11953Rabbit polyclonal IgG1 : 200
FSTL1AbcamAb71548Rabbit polyclonal IgG1 : 1000
ActinAnboC0124Rabbit polyclonal IgG1 : 5000