N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide
Figure 1
Cell viability of HepG2 cells assayed by MTT. The results are expressed as percentage of control, and each value represents the mean ± SD of six independent experiments. The annotation indicates a value < 0.01 versus untreated group. The annotation indicates a value < 0.05 versus H2O2 group. The annotation indicates a value < 0.01 versus H2O2 group. (a) Cell viability of HepG2 cells following different concentrations of H2O2 exposure were measured by MTT assay. (b) Cytotoxicity of NAS to HepG2 cells was measured by MTT assay. HepG2 cells were treated by 200 μM H2O2 with or without a series of concentrations of NAS (1, 2.5, 5, 7.5, 10, 20, 30, 50, and 100 μM) for 6 hours. (c) The effect of NAS on HepG2 cell viability in response to H2O2 and comparing it with melatonin (grey bar), a well standardized antioxidant.
