N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide

Figure 2

NAS reduces H₂O₂-induced reactive oxygen species production. (A) Intracellular ROSdetected by flow cytometry afterH2DCF staining. (B) MDA content (white bars) and SOD activity (black bars) were measured in HepG2 cell lysates. (a) Untreated group; (b) H₂O₂ group, HepG2 cells incubated with 200 μM H₂O₂ for 6 hours; (c) H₂O₂ + NAS group, 10 μM NAS was administered before subjecting to H₂O₂. The significant increase in MDA levels and decrease in SOD levels in the H₂O₂ group compared with untreated group are shown (𝑃<0.01). NAS attenuated the H₂O₂-induced changes in MDA and SOD (𝑃<0.01). Error bars represent SD (𝑛=3). The annotation ∗∗indicates a 𝑃 value < 0.01 versus untreated group. The annotation ##indicates a 𝑃 value < 0.01 versus H₂O₂ group. Experiments were performed at least three times.