Saponins from Aralia taibaiensis Attenuate D-Galactose-Induced Aging in Rats by Activating FOXO3a and Nrf2 Pathways

Figure 5

Saponins supplementation activated FOXO3a pathway in liver of D-galactose-induced aging rats. The protein levels of SOD2, CAT, and SOD1 were determined by Western blot (a). The transcriptional activity of FOXO3a was determined by EMSA (b). The protein levels of p-FOXO3a (S253) (c) and p-Akt (d) were also determined by Western blot. The band intensities were quantified by Quantity One Software version 4.6.3. Protein levels were normalized to β-actin as compared with the untreated Control (set to 1). Data are expressed as mean ± SD; 𝑛≥3. Intergroup comparisons were performed by ANOVA. ^∗𝑃<0.05 versus Control and ^#𝑃<0.05 versus D-gal.

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