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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 329172, 10 pages
http://dx.doi.org/10.1155/2014/329172
Research Article

Anticonvulsant and Antioxidant Effects of Tilia americana var. mexicana and Flavonoids Constituents in the Pentylenetetrazole-Induced Seizures

1Laboratory of Neurochemistry, National Institute of Pediatrics, 04530 Mexico City, DF, Mexico
2Laboratory of Neuropharmacology of Natural Products, National Institute of Psychiatry, 14370 Mexico City, DF, Mexico
3Ecology and Natural Resources Department, Faculty of Sciences, National Autonomous University of Mexico, 04150 Mexico City, DF, Mexico
4Neurology Service, National Institute of Pediatrics, 04530 Mexico City, DF, Mexico
5Laboratory of Molecular Biology and Genomic, Faculty of Sciences, National Autonomous University of Mexico, 04150 Mexico City, DF, Mexico

Received 8 February 2014; Accepted 29 June 2014; Published 13 August 2014

Academic Editor: Rivelilson Mendes de Freitas

Copyright © 2014 Noemí Cárdenas-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tilia genus is commonly used around the world for its central nervous system properties; it is prepared as tea and used as tranquilizing, anticonvulsant, and analgesic. In this study, anticonvulsant activity of the Tilia americana var. mexicana inflorescences and leaves was investigated by evaluating organic and aqueous extracts (100, 300, and 600 mg/kg, i.p.) and some flavonoids in the pentylenetetrazole-induced seizures in mice. Moreover, antioxidant effect of these extracts and flavonoids was examined in an in vitro study by using spectrophotometric technique. Significant activity was observed in the methanol extract from inflorescences. An HPLC analysis of the methanol extract from inflorescences and leaves of Tilia allowed demonstrating the respective presence of some partial responsible flavonoid constituents: quercetin (20.09 ± 1.20 μg/mg and 3.39 ± 0.10 μg/mg), rutin (3.52 ± 0.21 μg/mg and 8.94 ± 0.45 μg/mg), and isoquercitrin (1.74 ± 0.01 μg/mg and 1.24 ± 0.13 μg/mg). In addition, significant but different antioxidant properties were obtained among the flavonoids and the extracts investigated. Our results provide evidence of the anticonvulsant activity of Tilia reinforcing its utility for central nervous system diseases whose mechanism of action might involve partial antioxidant effects due to the presence of flavonoids.