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Oxidative Medicine and Cellular Longevity
Volume 2014 (2014), Article ID 376515, 7 pages
Clinical Study

Aging Aggravates Nitrate-Mediated ROS/RNS Changes

1Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China
2Department of Neurology, The Second Affiliated Hospital of Chong Qing Medical University, Chong Qing, China
3Department of Emergency Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
4Heart Center, Beijing Chaoyang Hospital-Affiliate of Beijing Capital Medical University, 8 Gongtinan Road, Beijing 100020, China

Received 2 August 2013; Revised 17 August 2013; Accepted 18 August 2013; Published 23 March 2014

Academic Editor: Zhengyuan Xia

Copyright © 2014 Qian Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nitrates are the most frequently prescribed and utilized drugs worldwide. The elderly are a major population receiving nitrate therapy. Both nitrates and aging can increase in vivo reactive oxygen species (ROS) and reactive nitrogen species (RNS). To date, the effects of aging upon nitrate-induced ROS/RNS alteration are unknown. The present study tested the effects of aging upon nitrate-induced ROS/RNS alteration in vivo. 32 adults and 43 elderly unstable angina (UA) patients were subjected to 48 hours of isosorbide dinitrate intravenous injection (50 μg/minutes) in this clinical study. Blood samples were obtained at baseline and conclusion. Outcome measures of oxidative stress included plasma malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione (GSH). Plasma concentrations of NOx and nitrotyrosine served as markers of RNS. Because of the significant differences in basic clinical characters between adults and the elderly, we designed an additional experiment determining ROS/RNS stress in rat cardiac tissue. Additionally, rat thoracic aortic NOS activity served as a marker indicating endothelial function. Our study demonstrated that nitrate therapy significantly increased in vivo ROS/RNS stress in the elderly compared to adult patients, confirmed by animal data. Decreased NOS activity was observed in old rats. Taken together, the present study’s data suggests a synergism between nitrate treatment and the aging process.