Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons

Figure 6

Acridine orange detection of acidic lysosomes. Acridine orange enters acidic compartments such as lysosomes and becomes protonated and sequestered. (a) Microphotographs illustrating an intense fluorescence when CuE is administered alone and low of fluorescence in MPP⁺ or CuE + MPP⁺ conditions. Nuclei were counterstained in blue with Hoechst 33342. Scale bar = 10 μm. (b) Histogram. Semiquantitative measures of acridine orange fluorescence. Data are expressed as relative fluorescence units and are means ± S.E.M. . versus Ctrl.

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