A Novel Contrast-Induced Acute Kidney Injury Model Based on the 5/6-Nephrectomy Rat and Nephrotoxicological Evaluation of Iohexol and Iodixanol In Vivo

Figure 8

Urine volume, renal hypoxic conditions, and immunofluorescent labeling for TUNEL in the control, iohexol, and iodixanol groups. (a) Urine volume of rats in the three groups (recorded from the start of the bolus injection to 15 min or 30 min after the injection). The urine volume of rats in the iohexol group is more than that in the iodixanol group. (b) Quantitative analysis of the extent and intensity of renal Hypoxyprobe in the three groups. A little staining in the representative immunostaining of the control group, more staining in the representative immunostaining of the iohexol group compared with the control group, and less staining in the representative immunostaining of the iodixanol group. (c) Representative photomicrographs of Hypoxyprobe (anti-pimonidazole protein adducts antibody) immunostaining in renal sections 15 min and 30 min after a saline, iohexol, or iodixanol injection. Brown color indicates positive staining (arrow). Original magnifications: ×200. Calibration bar = 20 μm. versus the control group; . Data are means ± SD.

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