A Novel Contrast-Induced Acute Kidney Injury Model Based on the 5/6-Nephrectomy Rat and Nephrotoxicological Evaluation of Iohexol and Iodixanol In Vivo

Figure 9

Immunofluorescent labeling for TUNEL in rat kidney tissue sections of the control, iohexol, and iodixanol groups. (a) Representative photomicrographs of TUNEL-positive cells in renal sections. Rat kidney tissue was stained for TUNEL (green). Nuclei were stained with DAPI (blue). Few TUNEL-positive cells in the representative immunofluorescent staining of the control group. A large number of TUNEL-positive cells in the representative immunofluorescent staining of iohexol group compared with control group, and less TUNEL-positive cells in the representative immunofluorescent staining of the iodixanol group. (b) Quantitative analysis of TUNEL-positive cells in the three groups by the percentage of TUNEL-positive cells. Original magnifications: ×630. Calibration bar = 20 μm. versus the control group; . Data are means ± SD.

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