Exercise Training Preserves Ischemic Preconditioning in Aged Rat Hearts by Restoring the Myocardial Polyamine Pool

Figure 2

(a) Infarct size was measured using tetrazolium chloride (TTC) staining to distinguish necrotic from viable myocardium. (b) Infarct size (expressed as the percentage of risk zone of the total left ventricle [LV]). (c) Lactate dehydrogenase (LDH) released from the coronary effluent in the different groups. The data of infarct size and LDH are presented as the mean ± SE ( per group). versus the YC group; ^† versus the YPC group; versus the Ex-OC group; ^▲ versus the Ex-OPC group. ((d)–(h)) The changes in various hemodynamic parameters following reperfusion for 30 min are shown for the young IR control group (YC), young preconditioning group (YPC), old IR control group (OC), old preconditioning group (OPC), exercised old IR control group (Ex-OC), exercised old preconditioning group (Ex-OPC), and DFMO treatment groups (DFMO was administered to the Ex-OC and Ex-OPC groups, resp., presented as the Ex-OC-I and the Ex-OPC-I). Data are presented as the mean ± SE ( per group). versus the YC group; ^† versus the YPC group; versus the Ex-OC group; versus Ex-OPC group. LVDP, left ventricular developed pressure; LVEDP, left ventricular end-diastolic pressure; , maximal positive and negative change in the left ventricular pressure; RPP, rate pressure product.

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