Exercise Training Preserves Ischemic Preconditioning in Aged Rat Hearts by Restoring the Myocardial Polyamine Pool

Table 2

Changes in ODC and SSAT enzyme activity and polyamine levels in rat cardiac tissue.

Groups	ODC activity	SSAT activity	Putrescine	Spermidine	Spermine
	nmol CO2 released/h/mg pro	nmol acetyl-spd formed/min/mg pro	nmol/g tissue wet weight
YC	16.25 ± 2.11	6.89 ± 1.18	8.03 ± 2.84	189.13 ± 13.80	186.72 ± 10.27
YPC	21.54 ± 2.6	4.13 ± 0.	4.56 ± 1.9	233.90 ± 17.5	208.06 ± 15.
OC	11.94 ± 1.82	9.99 ± 0.97	9.97 ± 2.02	121.14 ± 16.67	148.59 ± 14.76
OPC	12.31 ± 1.6	9.17 ± 1.	7.69 ± 1.	108.14 ± 15.7	132.44 ± 13.8
Ex-OC	13.01 ± 1.38	7.08 ± 0.86	7.25 ± 1.97	129.87 ± 13.73	130.03 ± 11.61
Ex-OPC	18.09 ± 1.6	4.09 ± 0.	4.07 ± 1.	156.03 ± 16.	169.81 ± 15.
Ex-OC-I	10.86 ± 1.	6.58 ± 0.69	4.01 ± 1	87.76 ± 16.	110.58 ± 13.
Ex-OPC-I	10.91 ± 1.	5.11 ± 0.88	2.33 ± 0.5	98.21 ± 15.8	124.71 ± 15.

Treatment groups are YC, OC and Ex-OC groups, isolated perfused young, old, and old exercised rat heart with IR treatment; YPC, OPC and Ex-OPC groups, isolated perfused young, old, and old exercised rat heart with IPC treatment; and Ex-OC-I and Ex-OPC-I groups, ODC inhibitor DFMO was given to both the Ex-OC and Ex-OPC heart before prolonged ischemia, respectively. Data are the mean ± SE, = 8 per group. , versus the YC group; , versus the YPC group; , versus the Ex-OC group; , versus the Ex-OPC group.