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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 504953, 10 pages
Research Article

Insulin Regulates Glucose Consumption and Lactate Production through Reactive Oxygen Species and Pyruvate Kinase M2

1Department of Pathology, State Key Lab of Reproductive Medicine, Cancer Center, Nanjing Medical University, Nanjing 210029, China
2Department of Pathology, Anhui Medical University, Hefei 230032, China
3Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
4Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA

Received 12 February 2014; Accepted 11 April 2014; Published 8 May 2014

Academic Editor: Jinxiang Zhang

Copyright © 2014 Qi Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

MiR-128 and MiR-145 Are Inhibited by ROS. There is accumulating evidence for the miRNA expression which may be altered in response to exogenous agents that, at least in part, induce intracellular insulin and oxidative stress [21, 24]. We treated hepatocellular carcinoma cells using hydrogen peroxide, and found that miR-128 and miR-145 expression levels were inhibited by hydrogen peroxide in both HepG2 and Bel7402 cells (Figure S1). This result suggests that ROS inhibit miR-128 and miR-145 expression.

Supplementary figure legend: FIGURE.S1. HepG2 cells and Bel7402 cells were cultured in serum-free medium overnight. The cells were treated with 50 µM H2O2 for 6 h. Total RNAs were extracted and used for real time RT–PCR analysis for detecting the expression levels of miR-145, miR-128 and U6. ** indicates significant difference compared to the control (p< 0.01).

  1. Supplementary Materials