RCAN1 Regulates Mitochondrial Function and Increases Susceptibility to Oxidative Stress in Mammalian Cells
Figure 5
RCAN1 overexpression increases mitochondrial ROS production. Representative images of (a) control and (b) cells stained with 100 μM MitoSOX Red at 20x magnification (scale bar = 50 μm). (c) Quantification of MitoSOX fluorescence intensity revealed that control cells (light bars) have significantly less mean fluorescence compared to cells (dark bars) under basal conditions. The addition of the antioxidant N-acetylcysteine (NAC) significantly reduces florescence in cells while having no effect on control cells. Data has been normalized to basal mean fluorescence in control cells and error bars represent SEM. cells from each group; .
