Multimarker Screening of Oxidative Stress in Aging

Table 6

HPLC elution program. Solvent A: water solution of ammonium hydroxide (pH 10.5); solvent B: solution of methanol : acetonitrile (60 : 40, v/v) with 0.1% of ammonium hydroxide.

Time [min]	Solvent A [%]	Solvent B [%]
0:00	70	30
10:00	70	30
25:00	5	95
30:00	5	95
32:00	70	30
40:00	70	30