Research Article

Effects of Treating Old Rats with an Aqueous Agaricus blazei Extract on Oxidative and Functional Parameters of the Brain Tissue and Brain Mitochondria

Figure 3

Reactive oxygen species (ROS) levels of brain homogenate and brain mitochondria from young, old, and A. blazei-treated old rats. The brain homogenate and the mitochondria were prepared as described in Section 2. The ROS levels were evaluated by means of the dichlorofluorescein diacetate method. The bars represent the mean ± mean standard errors of 5 (young), 7 (old), and 6 (A. blazei-treated) rats for the homogenate and of 4 (young), 6 (old), and 5 (A. blazei-treated) rats for the mitochondria. for the comparison between (1) and (2) and (2) and (3), according to ANOVA followed by Student-Newman-Keuls post hoc testing; for the comparison between (3) and (1); # for the comparison between (2) and (1).
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