Effects of Treating Old Rats with an Aqueous Agaricus blazei Extract on Oxidative and Functional Parameters of the Brain Tissue and Brain Mitochondria
Figure 4
Reduced glutathione (GSH) levels and protein thiol groups of brain homogenate and reduced glutathione (GSH) levels of brain mitochondria from young, old, and A. blazei-treated old rats. The brain homogenate and the mitochondria were prepared as described in Section 2. The GSH levels were evaluated spectrofluorimetrically by o-phthalaldehyde method. The reduced thiol groups were measured with 5,5′-dithiobis 2-nitrobenzoic acid (DTNB) method. The bars represent the mean ± mean standard errors; in panel (a) (tissue homogenate) the values were 5 (young), 9 (old), and 6 (A. blazei-treated); in panel (b) the values were 6 (young), 6 (old), and 6 (A. blazei-treated) and in panel (c) values were 5 (young), 6 (old), and 5 (A. blazei-treated). for the comparison between (1) and (2) and (2) and (3), according to ANOVA followed by Student-Newman-Keuls post hoc testing.
