Effects of Treating Old Rats with an Aqueous Agaricus blazei Extract on Oxidative and Functional Parameters of the Brain Tissue and Brain Mitochondria

Table 2

Effects of the A. blazei extract treatment on several enzymatic activities of brain mitochondrial from old rats. All enzymes were evaluated spectrophotometrically as described in Materials and Methods. The values represent the mean ± standard errors of the mean.

Enzymes	Experimental groups
	(1) Young rats	(2) Old rats	(3) A. blazei-treated old rats
nmol min^−1 mg protein^−1
NADH dehydrogenase	698.90±29.99 (𝑛=3)	588.17±16.51^∗ (𝑛=5)	752.12±36.35 (𝑛=6)
Succinate dehydrogenase	14.22±1.84 (𝑛=6)	7.83±0.94^∗ (𝑛=5)	14.57±0.95 (𝑛=6)
𝛼-Ketoglutarate dehydrogenase	17.24±1.48 (𝑛=6)	10.98±0.54^∗ (𝑛=5)	16.13±0.98 (𝑛=6)
Pyruvate dehydrogenase	23.75±2.37 (𝑛=4)	17.26±0.27^# (𝑛=5)	21.03±1.22 (𝑛=6)
Cytochrome c oxidase	287.20±31.30 (𝑛=5)	264.53±16.23 (𝑛=5)	501.32±25.09^∗∗ (𝑛=4)
Malate dehydrogenase	7540±560 (𝑛=6)	6780±770 (𝑛=5)	6980±270 (𝑛=6)
Isocitrate dehydrogenase	26.68±1.79 (𝑛=6)	25.80±2.89 (𝑛=5)	27.57±1.31 (𝑛=6)
Glutamate dehydrogenase	183.6±11.9 (𝑛=6)	179.5±19.9 (𝑛=5)	179.9±8.4 (𝑛=6)

^∗𝑃<0.05 for (2) versus (1) and for (2) versus (3) (ANOVA and Student-Newman-Keuls post hoc testing).
^#𝑃<0.05 for (2) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).
^∗∗𝑃<0.05 for (3) versus (2) and (3) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).