Effects of Treating Old Rats with an Aqueous Agaricus blazei Extract on Oxidative and Functional Parameters of the Brain Tissue and Brain Mitochondria

Table 5

Effects of the A. blazei extract treatment on the respiration of brain mitochondria of old rats. The oxygen consumption, ADP/O ratio, and respiration coefficient (RC) were assessed in the mitochondria as described in Materials and Methods. The values represent the mean ± standard errors of the mean.

Substrate	Experimental groups	Rate of oxygen uptake; (nmol min^−1 mg protein^−1)		ADP/O	RC
		−ADP	+ADP
Succinate (10 mM)	(1) Young rats	16.11±1.27 (𝑛=6)	51.65±2.06 (𝑛=6)	1.54±0.02 (𝑛=6)	2.67±0.14 (𝑛=6)
	(2) Old rats	14.16±1.477 (𝑛=6)	41.6±2.68^∗ (𝑛=6)	1.22±0.14 (𝑛=5)	2.30±0.32 (𝑛=6)
	(3) A. blazei-treated old rats	16.01±0.976 (𝑛=7)	52.20±3.50 (𝑛=6)	1.24±0.16 (𝑛=6)	2.54±0.27 (𝑛=6)
Pyruvate + L-malate; (10 mM + 1 mM)	(1) Young rats	9.95±0.26 (𝑛=6)	40.47±2.44 (𝑛=6)	1.78±0.10 (𝑛=6)	1.92±0.10 (𝑛=6)
	(2) Old rats	9.64±0.71 (𝑛=5)	30.71±3.03^# (𝑛=5)	1.66±0.28 (𝑛=5)	1.85±0.16 (𝑛=5)
	(3) A. blazei-treated old rats	10.64±0.54 (𝑛=7)	35.48±2.12 (𝑛=7)	1.67±0.04 (𝑛=6)	1.95±0.18 (𝑛=7)
α-Keto-glutarate (10 mM)	(1) Young rats	4.85±0.46 (𝑛=6)	18.50±1.39 (𝑛=6)	1.88±0.08 (𝑛=6)	2.63±0.39 (𝑛=6)
	(2) Old rats	2.99±0.62^# (𝑛=3)	15.53±1.53 (𝑛=3)	2.17±0.21 (𝑛=3)	3.22±0.90 (𝑛=3)
	(3) A. blazei-treated old rats	3.51±0.15 (𝑛=3)	16.39±0.81 (𝑛=3)	2.60±0.12^∗∗ (𝑛=3)	4.43±2.29 (𝑛=3)

^∗𝑃<0.05 for (2) versus (1) and (2) versus (3) (ANOVA and Student-Newman-Keuls post hoc testing).
^#𝑃<0.05 for (2) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).
^∗∗𝑃<0.05 for (3) versus (1) (ANOVA and Student-Newman-Keuls post hoc testing).