Resveratrol Inhibits Phenotype Modulation by Platelet Derived Growth Factor-bb in Rat Aortic Smooth Muscle Cells
Figure 5
The effects of resveratrol on modulation of PDGF-bb-stimulated signaling pathways in RAOSMCs. RAOSMCs starved of serum were stimulated with 10 ng/mL PDGF-bb and 100 μM resveratrol for the indicated times (10 m, 30 m, 1 h, 2 h, and 4 h) and lysed. Lysates were immunoblotted with antibodies. After densitometric quantification using the ImageJ program, data were expressed each as the mean SD from three independent experiments. Black bar indicates expression by PDGF-bb stimulation. White bar indicates expression by PDGF-bb stimulation with EGCG. (a) The expression of phospho-PDGFR- in a time-dependent manner. The band intensities were normalized to PDGFR- expression. (b) The time-dependent expressions of phospho-MEK1/2 and phospho-p42/44MAPK. The band intensities were normalized to MEK1/2 and p42/44MAPK expression. (c) The time-dependent expression of phospho-p38 MAPK. The band intensities were normalized to p38 MAPK expression. (d) The time-dependent expression of phospho-Akt and phospho-mTOR. The band intensities were normalized to Akt and mTOR expression.
