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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 579305, 9 pages
http://dx.doi.org/10.1155/2014/579305
Research Article

Antiosteoporotic Effect of Combined Extract of Morus alba and Polygonum odoratum

1Department of Physiology and Graduate School (Neuroscience Program), Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand
2Integrated Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40000, Thailand
3Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand

Received 7 August 2014; Revised 11 October 2014; Accepted 15 October 2014; Published 12 November 2014

Academic Editor: Liang-Jun Yan

Copyright © 2014 Sudarat Sungkamanee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Due to the limitation of osteoporosis therapy, the alternative therapies from natural sources have been considered. In this study, we aimed to determine the antiosteoporotic effect of the combined extract of Morus alba and Polygonum odoratum leaves. Ovariectomized rats, weighing 200–220 g, were orally given the combined extract at doses of 5, 150, and 300 mg·kg−1 BW for 3 months. At the end of study, blood was collected to determine serum osteocalcin, calcium, and alkaline phosphatase level. In addition, tibia bone was isolated to determine bone oxidative stress markers, cortical bone thickness, and density of osteoblast. The combined extract decreased oxidative stress and osteoclast density but increased osteoblast density and cortical thickness. The elevation of serum calcium, alkaline phosphatase, and osteocalcin was also observed. These results suggested the antiosteoporotic effect of the combined extract via the increased growth formation together with the suppression of bone resorption. However, further studies concerning chronic toxicity and the underlying mechanism are required.