Lentivirus-Mediated Nox4 shRNA Invasion and Angiogenesis and Enhances Radiosensitivity in Human Glioblastoma
Figure 6
Nox4 silencing inhibited tube-like structure formation of endothelial cells and reduced VEGF expression in glioblastoma cells. (a) Tube-like structure formation ability of human umbilical veins endothelial cells (HUVECs) that were cocultured with conditioned medium derived from Nox4-shRNA or scrambled U87 cells, treated with or without 4 Gy irradiation, was measured. After incubation, endothelial cells were fixed, and tube-like structures were photographed (magnification, ×100). (b) Effects of Nox4-shRNA on expression of VEGF in U87MG and U251 cells. Cells were infected with Nox4-shRNA or scrambled control and treated with or without 4 Gy irradiation. Samples were collected at 24 h postradiation. VEGF protein levels in the culture supernatant were determined by ELISA. Columns, mean; bars, SD; RT, radiation therapy; *, versus scrambled; @, versus scrambled and scrambled + RT.
