High-Dialysate-Glucose-Induced Oxidative Stress and Mitochondrial-Mediated Apoptosis in Human Peritoneal Mesothelial Cells

Figure 4

NAC, an ROS scavenger, reduced high-dialysate-glucose-induced ROS generation and oxidative damage in HPMCs. HPMCs were pretreated with 1 or 5 mM NAC for 1 h before glucose stimulation. The levels of cellular ROS (a), lipid peroxides (b), and mitochondrial superoxides (c) were detected using flow cytometry. Pretreatment with 5 mM NAC reduced high-dialysate-glucose-induced oxidative insults. (d) The fluorescent intensity of mitochondrial superoxide and mitochondrial morphology using MitoSOX Red were detected by confocal microscopy. Antioxidant NAC reduced mitochondrial superoxide generation and rescued mitochondria from extensive fragmentations.