Piperlongumine Inhibits Migration of Glioblastoma Cells via Activation of ROS-Dependent p38 and JNK Signaling Pathways
Figure 6
Effects of PL on NFκB nuclear translocation and IκBα expression in LN229 cells. (a) Representative western blots of NFκB and IκBα at 3 h after scratching in LN229 cells. Cells were pretreated with PL for 6 h; NAC, SB203580, or SP600125 was administrated 2 h before PL treatment. Cytosolic (cyto) and nuclear (nuc) protein were isolated and subjected to western blot analysis. β-actin was set as loading control. (b) Statistical analysis of nuc/cyto NFκB of western blot results. Statistical analysis of IκBα expression of western blot results. compared to group of 0 μM of PL; compared to group of 20 μM of PL treatment alone.
