Mitochondria-Targeted Antioxidant Prevents Cardiac Dysfunction Induced by Tafazzin Gene Knockdown in Cardiac Myocytes
Figure 1
Tafazzin shRNA adenovirus knocked down tafazzin expression and cardiolipin. NVMs were transduced with the tafazzin (TazD) shRNA adenovirus for 48 h and tested for tafazzin expression ((a) and (b)) and cardiolipin ((c), (d), and (e)). (a) Tafazzin mRNA was measured by real-time RT-PCR using β-actin as a normalizer and expressed as relative level compared to the control (scrambled adenovirus or SCR). Data represent mean ± SE of 4 separate experiments. versus SCR. (b) Tafazzin protein was determined by Western blot and expressed as a percentage of SCR. Data represent mean ± SE of 3 separate experiments. versus SCR. (c) Cardiolipin (CL) and monolysocardiolipin (lyso-CL) were identified and quantified by mass spectrometry. Data represent mean ± SE of 9 separate experiments. versus SCR. The basal contents (including all detected species) of cardiolipin and monolysocardiolipin were and nmol/mg protein, respectively. Species of cardiolipin (d) and monolysocardiolipin (e) were identified and quantified by shotgun lipidomics. Data represent mean ± SE of 8 separate experiments. versus SCR.
