Mitochondria-Targeted Antioxidant Prevents Cardiac Dysfunction Induced by Tafazzin Gene Knockdown in Cardiac Myocytes
Figure 3
Mito-Tempo abolished ATP shortage induced by tafazzin knockdown. (a) C2C12 cells were cultured in high glucose DMEM to 70% confluence, serum starved, and treated with 5 nM rotenone (ROT) in glucose-free or high glucose DMED for 24 h. Data represent mean ± SE of 3 separate experiments. versus control cells which were treated with DMSO. (b). NVMs were transduced with the shRNA adenovirus for 48 h, treated with mito-Tempo in the last 24 h, and tested for ATP with a kit from BioVision. Data represent mean ± SE of 4 separate experiments. versus SCR treated with vehicle. versus Taz knockdown cells treated with vehicle.
