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Oxidative Medicine and Cellular Longevity
Volume 2014, Article ID 657512, 9 pages
Research Article

Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H2O2

1Medical Sciences Division, Northern Ontario School of Medicine, Laurentian University, Sudbury, ON, Canada P3E 2C6
2Department of Biology, Laurentian University, Sudbury, ON, Canada P3E 2C6
3Department of Gerontology, Huntington University, Sudbury, ON, Canada
4Department of Chemistry and Biochemistry, Laurentian University, Sudbury, ON, Canada P3E 2C6
5Biomolecular Sciences Program, Laurentian University, Sudbury, ON, Canada P3E 2C6

Received 12 August 2013; Revised 15 October 2013; Accepted 11 November 2013; Published 12 January 2014

Academic Editor: Adriane Belló-Klein

Copyright © 2014 Sandhya Khurana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Reactive oxygen species trigger cardiomyocyte cell death via increased oxidative stress and have been implicated in the pathogenesis of cardiovascular diseases. The prevention of cardiomyocyte apoptosis is a putative therapeutic target in cardioprotection. Polyphenol intake has been associated with reduced incidences of cardiovascular disease and better overall health. Polyphenols like epigallocatechin gallate (EGCG) can reduce apoptosis of cardiomyocytes, resulting in better health outcomes in animal models of cardiac disorders. Here, we analyzed whether the antioxidant N-acetyl cysteine (NAC) or polyphenols EGCG, gallic acid (GA) or methyl gallate (MG) can protect cardiomyocytes from cobalt or H2O2-induced stress. We demonstrate that MG can uphold viability of neonatal rat cardiomyocytes exposed to H2O2 by diminishing intracellular ROS, maintaining mitochondrial membrane potential, augmenting endogenous glutathione, and reducing apoptosis as evidenced by impaired Annexin V/PI staining, prevention of DNA fragmentation, and cleaved caspase-9 accumulation. These findings suggest a therapeutic value for MG in cardioprotection.