Effect of Staurosporine in the Morphology and Viability of Cerebellar Astrocytes: Role of Reactive Oxygen Species and NADPH Oxidase

Figure 8

Cytoskeletal rearrangements induced by St are not mediated by ROS. Cerebellar astrocytes were treated with St (0.5 μM) for 2 h in the presence or absence of the antioxidants MnTMPyP (50 μM) and Euk-134 (20 μM) and the NOX inhibitors DPI (520 nm) and AEBSF (50 μM). Immunostaining against β-tubulin (green) and staining with rhodamine-phalloidin (red) were performed as mentioned in Section 2. Scale bar represents 50 μM.