Effect of Staurosporine in the Morphology and Viability of Cerebellar Astrocytes: Role of Reactive Oxygen Species and NADPH Oxidase
Figure 9
St does not induce changes in the expression of cytoskeletal proteins. Actin and α-tubulin levels were determined by Western blot assays as detailed in Section 2. Cerebellar astrocytes were treated with St (0.5 μM) in the presence or absence of the antioxidants MnTMPyP (50 μM) and Euk-134 (20 μM) and the NOX inhibitors DPI (520 nm) and AEBSF (50 μM). Data are presented as mean ± SEM of three independent experiments. No statistical differences were found among the treatments.
