Effects of Nrf2 Deficiency on Bone Microarchitecture in an Experimental Model of Osteoporosis
Osteoclast differentiation of bone marrow-derived cells. Cells were cultured in complete DMEM medium containing M-CSF (30 ng/mL) and RANKL (50 ng/mL). TRAP staining was performed after 8 days of culture and TRAP+ multinucleated cells were counted. (a) Representative images, original magnification ×400. OVX: ovariectomy. (b) Cell quantification. Data represent mean ± SD (). , .