PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death

Figure 5

Effect of PRAK on DJ-1 nuclear localization under oxidative stress. (a) PRAK^+/+ cells synchronized by serum starvation for 48 hrs and treated with culture medium (upper) or 300 μM H₂O₂ (lower) for 6 hrs were stained with anti-DJ-1 antibody. (b) PRAK^−/− cells synchronized by serum starvation for 48 hrs and treated with culture medium (upper) or 300 μM H₂O₂ (lower) for 6 hrs were stained with anti-DJ-1 antibody. Nuclei were stained with DAPI. Scale bar = 20 μm. (c) and (d). The nuclear and cytoplasmic fluorescence intensities of DJ-1 in PRAK^+/+ cells (c) and PRAK^−/− cells (d) were analyzed. Data are expressed as the mean ± SD of four separate experiments. compared with DJ-1 in the nucleus of control or H₂O₂-treated PRAK^+/+ cells (c); compared with DJ-1 in the nucleus of control PRAK^−/− cells (d).

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