PRAK Interacts with DJ-1 and Prevents Oxidative Stress-Induced Cell Death

Figure 6

PRAK phosphorylates DJ-1 both in vitro and in vivo. (a) GST-tagged DJ-1 was coincubated with His-tagged PRAK or His-tagged p38 and further analyzed by Western blot. (b) The relative intensities of phosphorylated DJ-1 were analyzed and data are expressed as the mean ± SD of three separate experiments. compared with GST-DJ-1 coincubated with His-p38. (c) PRAK^+/+ and PRAK^−/− cells were treated with 300 μM H₂O₂ for different time periods. The expression of phosphorylated and total DJ-1 was assessed by Western blot analysis. (d) The relative intensities of phosphorylated DJ-1 were analyzed and data are expressed as the mean ± SD of three separate experiments. compared with PRAK^−/− cells.

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