Elimination of serum atherogenicity with LDL-agarose column. Five milliliters of the serum was passed through the LDL-sepharose column at a flow rate of 1 mL/min for 30 min. The sorbent was then eluted with 2 mL glycine buffer (pH 2.7), and the eluate was dialyzed against a 2,000-fold excessive volume of medium 199 for 24 hours at 4°C. The cells were cultured in the presence of the initial or treated serum and with the proper volume of the dialyzed eluate.
