[Retracted] PKCδ Promotes High Glucose Induced Renal Tubular Oxidative Damage via Regulating Activation and Translocation of p66Shc

Figure 7

PKCδ siRNA attenuates the generation of intracellular ROS and mitochondrial superoxide in HK-2 cells exposure to HG. (a) HK-2 cells were stained with dichlorofluorescein-diacetate (DCFH-DA) and MitoSOX, respectively, and then examined by confocal microscopy. Results indicate that PKCδ siRNA exhibits reduced intracellular ROS production in HK-2 cells after treatment with HG (row 1). Similar results were seen in mitochondrial ROS production as assessed by MitoSOX staining (row 2). ((b) and (c)) The bar graphs represent a summary of flow cytometry analyses of cells stained with DCFH-DA and MitoSOX, respectively. Values are means ± SEM, . versus 5 mM D-glucose. versus 30 mM D-glucose.

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