Nrf2 Deficiency Exaggerates Doxorubicin-Induced Cardiotoxicity and Cardiac Dysfunction

Figure 7

Effects of Nrf2, miNrf2, and Atg5 on Dox-induced cell death in cardiomyocytes. (a), Overexpression of Atg5 on Dox-induced accumulation of ubiquitinated proteins in Nrf2 depleted rat neonatal cardiomyocytes. Cells infected with mNrf2 were further infected with Ad-Gfp or Ad-Atg5 and then treated with Dox (1 μM) for 24 h. 𝑛=4, ^∗𝑃<0.05 versus Ad-miNrf2 + Ad-Gfp (−). (b) Rat neonatal cardiomyocytes infected with Ad-Gfp, Nrf2, Atgr5, Ad-scramble, and Ad-miNrf2 as indicated were treated with or without Dox (1 μM) for 24 h and cell death was assessed by LDH cell death assay kit. 𝑛=4, ^∗𝑃<0.05 versus Dox (0 μM) in the same experimental group.

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