The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro
Figure 1
(a) WST-1 test of nonsteatotic (NH) and steatotic rat hepatocytes (SH) in primary culture treated with 0.01–1 mM tBHP (NH + tBHP; SH + tBHP) for 60 min. The values are means ± SD (). Results are expressed in percent where 100% is the activity of cellular dehydrogenases in control NH. versus control NH; versus control SH; versus corresponding NH + tBHP. (b) Time course of LDH activity (IU/L) in media of lean (NH) and steatotic rat hepatocytes (SH) in primary cultures treated with 0.25 mM tBHP (NH + tBHP; SH + tBHP) for up to 60 min. The values are means ± SD (). versus control NH; and versus control SH at corresponding time; versus NH + tBHP at corresponding time. (c) Time and concentration course of ROS generation (CM-H2DCFDA) in nonfatty (NH) and steatotic rat hepatocytes (SH) in primary culture treated with 0.25–0.5 mM tBHP (NH + tBHP; SH + tBHP) for up to 60 min. The values are means ± SD (). Results are expressed in percent where 100% is production of ROS by control NH for each concentration of tBHP. values are not shown.
