The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro

Figure 3

(a) Production of ROS (CM-H2DCFDA) in nonsteatotic (NH) and steatotic rat hepatocytes (SH) treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25 mM tBHP (NH + tBHP; SH + tBHP), or with 5 𝜇M TFP and 0.25 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for 30 min. The values are means ± SD (𝑛=8). Results are expressed in percent where 100% is production of ROS by control NH. ^∗∗∗𝑃<0.001 versus control NH; ^&&𝑃<0.01 versus NH + tBHP; ^###𝑃<0.001 versus control SH; ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes; ^$$$𝑃<0.001 versus SH + tBHP. (b) Concentration of MDA (𝜇mol/L) in culture media of nonsteatotic (NH) and steatotic rat hepatocytes (SH) treated with 0.25 mM tBHP (NH + tBHP; SH + tBHP) or with 5 𝜇M TFP and 0.25 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for 30 min. The values are means ± SD (𝑛=6). ^∗𝑃<0.05 and ^∗∗∗𝑃<0.001 versus control NH; ^##𝑃<0.01 versus control SH; ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes. (c) Intracellular GSH to total glutathione ratio in nonsteatotic (NH) and steatotic rat hepatocytes (SH) treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25 mM tBHP (NH + tBHP; SH + tBHP), or with 5 𝜇M TFP and 0.25 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for 30 min. The values are means ± SD (𝑛=5). Results are expressed in percent of GSH from total glutathione. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 versus control NH; ^&&&𝑃<0.001 versus NH + tBHP0.375; ^#𝑃<0.05 and ^###𝑃<0.001 versus control SH; ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes.

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