The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro

Figure 5

Percentage of nonsteatotic (NH) and steatotic rat hepatocytes (SH) with energized mitochondria treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25, 0.375, and 0.5 mM tBHP, respectively (NH + tBHP; SH + tBHP), or with 5 𝜇M TFP and 0.25, 0.375, and 0.5 mM tBHP, respectively (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for a period of 30 min. The values are means ± SD (𝑛=16). ^∗∗∗𝑃<0.001 versus control NH; ^&&&𝑃<0.001 versus corresponding NH + tBHP group; ^###𝑃<0.001 versus control SH; ^xxx𝑃<0.001 versus corresponding NH + tBHP group; ^$$$𝑃<0.001 versus corresponding SH + tBHP group.