The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro

Table 2

Respiration of digitonin-permeabilized (10 𝜇g/mL) hepatocytes (125,000/mL) in K⁺-medium at a temperature of 30°C. State 4 respiration (10 mM glutamate + 2.5 mM malate) and state 3 respiration (10 mM glutamate + 2.5 mM malate + 1.5 mM ADP) were measured and respiratory control ratio (RCR, state 3/state 4) was calculated in control nonsteatotic (NH) and steatotic rat hepatocytes (SH) and in lean and fatty hepatocytes preincubated with 0.25 mM tBHP for 5 min (NH + tBHP; SH + tBHP). Oxygen uptake at state 3 and state 4 is expressed as pmoles oxygen per second per million cells. ^∗∗𝑃<0.01 and ^∗∗∗𝑃<0.001 versus NH; ^###𝑃<0.001 versus SH; ^$$𝑃<0.01 versus NH + tBHP.

	State 3	State 4	RCR of complex I
	(pmol O2/s/10^6 cells)	(pmol O2/s/10^6 cells)
NH (𝑛=11)	1015.5±145.1	188.5±44.5	5.4±1.2
NH + tBHP (𝑛=9)	761.0±145.6	233.0±61.6	3.3±0.7
	***	n.s. versus NH	***
SH (𝑛=10)	819.7±112.5	172.4±42.5	4.8±0.8
	**	n.s. versus NH	n.s. versus NH
SH + tBHP (𝑛=8)	520.4±98.2	217.6±76.0	2.4±0.6
	###	n.s. versus SH	###
	$$	n.s. versus NH + tBHP	n.s. versus NH + tBHP