Promoter Hypermethylation and Suppression of Glutathione Peroxidase 3 Are Associated with Inflammatory Breast Carcinogenesis
Figure 3
Gel electrophoresis of GPX3 methylation-specific PCR (GPX3-MSP) products. Representative results of MSP using (a) unmethylated primers and (b) methylated primers. M is the DNA marker; lanes N1 and N2 represent normal breast tissues, lanes 1–4 represent non-IBC carcinoma tissues, and lanes 4–8 represent IBC breast carcinoma tissues. (c) Bars represent intensity values of methylated/unmethylated (M/U) ratios as quantified by ImageJ software. Normal breast tissues () with an M/U ratio = 0 (no methylated bands were detected from MSP) were recognized as unmethylated. We detected a significant increase () in the M/U ratio in IBC () carcinoma tissues compared to non-IBC () carcinoma tissues.
