Increased Oxidative Stress Response in Granulocytes from Older Patients with a Hip Fracture May Account for Slow Regeneration

Figure 2

Aged granulocytes are more sensitive towards H₂O₂ induced toxicity as young granulocytes. (a) Levels of oxidative stress were determined by measuring lipid peroxidation in sera of young controls (YH), young patients with a fracture of the long bones (YF), elderly controls (OH), and elderly patients with a fracture of the long bones (OF). 𝑁=10 and 𝑛=2 per group. ^∗∗𝑃>0.01 and ^∗∗∗𝑃<0.001 as determined by one-way ANOVA. (b) Granulocytes from young controls (𝑁=5, 𝑛=4) and elderly controls (𝑁=5, 𝑛=4) were stimulated with various concentrations of H₂O₂ (0, 0.002, 0.004, 0.008, 0.016, 0.03, 0.06, 0.125, 0.25, 0.5, and 1%). After 1 h viability was assessed by resazurin conversion. EC_50/young=0.18% and EC_50/old=0.09% (𝑃<0.05 as determined by Student’s 𝑡-test).

(a)

(b)