Increased Oxidative Stress Response in Granulocytes from Older Patients with a Hip Fracture May Account for Slow Regeneration

Figure 5

Inverted p38 activation in serum-conditioned HL60 cells in comparison to granulocytes. (a) Representative Western blot for phospho-p38, phospho-ERK1/2, and phospho-JNK in HL60 cells conditioned with serum of young controls (YH), young patients with a fracture of the long bones (YF), elderly controls (OH), and elderly patients with a fracture of the long bones (OF). GAPDH was used as a loading control and for normalization. For intermembrane comparison HEK293T cells (+) were used. Densitometric analysis of all samples (𝑁=10, 𝑛=2/group) was performed to determine relative expression levels of (b) phospho-p38, (c) phospho-ERK1/2, and (d) phospho-JNK. ^∗𝑃>0.05, ^∗∗𝑃>0.01 and ^∗∗∗𝑃<0.001 as determined by one-way ANOVA.

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